G - Physics – 01 – N
Patent
G - Physics
01
N
150/11, 167/45
G01N 35/00 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 15/10 (2006.01) G01N 15/12 (2006.01) G01N 15/14 (2006.01)
Patent
CA 1311404
ABSTRACT OF THE DISCLOSURE A method and apparatus for automatically and rapidly, retrieving, counting and/or analyzing at least one selected white blood cell population and/or subset thereof of a whole blood sample or portion thereof. A volume of a biological med- ium containing the white blood cells is prepared and at least one reactant specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed in one or more steps to obtain the desired white blood cell population analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.
561091
Coulter Wallace H.
Kortright Kenneth H.
Paul Ronald
Rodriguez Carlos
Russell Thomas
Coulter International Corp.
Moffat & Co.
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