Automated immunoassay

Details Automated immunoassay Automated immunoassay

A - Human Necessities

61

B

167/37, 73/92

A61B 10/00 (2006.01) G01N 33/00 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01)

Patent

CA 1088421

ABSTRACT An automated immunoassay apparatus which comprises: means for time controlled incubation of a multiplicity of sample solutions, each of which comprise a mixture of (a) a sample, possibly containing an antigen or antibody intended to be assayed; (b) a solution of a known concentration of a tagged antigen or tagged antibody; and when assaying for antigen, optionally; (c) a solution of a known titer of an antibody reactable with said antigen; activating detector means for determining the presence of the incubated sample and for activating a timing means upon detection of said sample at a detection station; means for introducing said incubated sample into said acti- vating detector means; timing means, which is activatable by said activating detector means when said detector means detects the presence of the incubated sample at a detection station, for generating con- trol signals at predetermined time sequences; means for separating said measured solution into a first portion containing antigen or antibody which has not been complexed during the said incubation, and a second portion containing antigen or antibody which has been complexed during said incubation; means for introducing one of said portions from said sepa- rating means into a measuring detector means; measuring detector means for detecting the amount of tagged material in said portion while said portion is maintained in a virtually static condition; recording means coupled to said detector means for recording the amounts of tagged material in said measuring detector means; means for isolating said incubated sample from additional sample solutions introduced into said apparatus for assay, wherein said isolating means is activatable by a control signal emmitted from said timing means, whereby said isolating means isolates the system at a first predetermined signal fro said timing means, and whereby said isolating means reconnects the system to said incub- tion means at a second predetermined signal from said timing means, wherein the second predetermined time period is set so as to enable sufficient time to complete said measurement before the next incu- bated sample arrives in the said detector means for the next sequential measurement; and means for flushing said sample from said system after said measurement thereof so as to prepare said detector for the next sequential sample for said measurement.

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