Automated lead assay

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/527 (2006.01) C12Q 1/52 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/84 (2006.01)

Patent

CA 2178641

Detection of lead present in a sample, comprising the steps of: (a) adding a lead recovery agent to an assay solution containing lead from the sample, (b) adding to the assay solution a disulfide enzyme which is inhibited in the presence of lead; and (e) correlating the activity of the disulfide enzyme to the amount of lead in the sample. The lead recovery agent, preferably a lead chelating agent, enhances the sensitivity and accuracy of the assay such that the assay can be readily automated for detection of lead in whole blood using commercially available automation systems.

Un procédé de détection du plomb présent dans un échantillon consiste à: (a) ajouter un agent de récupération du plomb à une solution de titrage contenant du plomb provenant de l'échantillon; (b) ajouter à la solution de titrage une enzyme disulfure inhibée en présence de plomb; et (c) établir une corrélation entre l'activité de l'enzyme disulfure et la proportion de plomb dans l'échantillon. L'agent de récupération, de préférence un agent chélatant le plomb, accroît la sensibilité et la précision du titrage de sorte que le titrage peut être automatisé pour la détection du plomb dans le sang total, au moyen de systèmes d'automatisation disponibles dans le commerce.

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