Automated on-line evaporating light scattering detection to...

B - Operations – Transporting – 01 – D

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B01D 15/08 (2006.01) G01N 30/82 (2006.01) G01N 30/72 (2006.01) G01N 30/74 (2006.01) G01N 30/78 (2006.01) G01N 30/84 (2006.01)

Patent

CA 2356218

Single pass methods and systems for measuring the total masses of individual compounds present in fluid samples in real time, and particularly for fluid samples prepared in microtiter plate format are provided. Individual compounds are isolated by fluid separation column (14). Portions of the individual compounds isolated by column (14) are analyzed by mass spectrometer (16), which determines their concentration on the basis of their molecular weights, and an evaporative light scattering detector (24), (which determines the total masses of each of the isolated compounds passing therethrough). Total collected masses of the individual compounds are calculated by determining the amount and portion of the isolated compounds diverted into evaporative light scattering detector (24) during a period of fraction collection.

L'invention se rapporte à des procédés et à des systèmes uni-passe permettant de mesurer en temps réel les masses totales de composés individuels présents dans des échantillons fluides préparés dans un format de plaque de microtitrage. Les composés individuels sont isolés dans une colonne de séparation de fluides (14). Certaines parties de ces composés individuels sont analysées par le spectromètre de masse (16), ce qui permet de déterminer leur concentration en fonction de leurs poids moléculaires, et par un détecteur de diffusion de lumière à l'évaporation (24) (ce qui permet de déterminer les masses totales de chacun des composés isolés qui le traversent). On calcule les masses totales recueillies de ces composés individuels en déterminant la quantité et la fraction de ces composés isolés qui ont été déviés vers le détecteur de diffusion de lumière à l'évaporation (24) au cours d'une période de collecte de fractions.

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