Automated protein analyzer

Details Automated protein analyzer Automated protein analyzer

G - Physics

01

N

G01N 35/00 (2006.01) G01N 33/12 (2006.01) G01N 33/52 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2639152

An automated protein analyzer and method is disclosed. The protein analyzer includes means for reducing protein samples to small particles, a reaction vessel in material transfer communication with the homogenizer, a reservoir for binding dye composition in fluid communication with the reaction vessel, a metering pump in fluid communication with the reservoir and the reaction vessel for distributing discrete predetermined amounts of a binding dye composition to the reaction vessel, a filter in fluid communication with the reaction vessel for separating solids from filtrate after a dye binding reaction has taken place in the reaction vessel, and a colorimeter in fluid communication with the filter and the reaction vessel for measuring the absorbance of the filtrate from the reaction vessel and the filter. The method includes mixing a binding dye composition and a protein sample using a homogenizer, measuring a parameter selected -from the group consisting of the speed of the homogenizer and the resistance of the mixture to the homogenizer, adjusting the speed of the homogenizer based upon the measured parameter, pumping unreacted dye composition from the mixture and to a colorimeter, and measuring the absorbance of the dye composition in the colorimeter.

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