Automatic focus system

Details Automatic focus system Automatic focus system

G - Physics

02

B

G02B 7/09 (2006.01) G02B 7/28 (2006.01) G02B 21/00 (2006.01) G02B 21/24 (2006.01)

Patent

CA 2229175

An automated focus system comprising an intelligent controlled electro-mechanical actuation system for manoeuvring a microscope lens. The focus system brings a stained biological material into optimal focus for image acquisition. The automated focus system is operable without human intervention and utilizes a merit function based on the "texture" of a dark stained biological material in the field of view of the microscope objective. The system utilizes a maximization procedure using a feedback technique related to the merit function in order to control the position of the objective lens. The merit function, in turn, is based on a series of calculations performed on a set of digitized images captured at different focal positions. The use of an intelligent control routine to issue instructions to the motion control system allows the device to avoid the usual focus and pitfalls associated with microscopic image capture. The automated focus system also includes a focus mapping method which is particularly suited to monolayer specimens. The focus mapping method generates a mapping of the surface of the microscope slide which is then used to predict the optimal focus position at any point within the mapped region.

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