Automatic on-the-fly focusing for continuous image...

G - Physics – 02 – B

Patent

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G02B 21/00 (2006.01) G02B 21/24 (2006.01)

Patent

CA 2360936

The invention provides an apparatus, and related method, for continuous volume imaging a specimen using imaging a plurality of image planes at predetermined focal depths. The focal depth for each image plane is adjustable in accordance with a variety of desired spatial configurations. The images generated at the image planes are processing in parallel and the image corresponding the an optimal focal depth at a specimen location is selected for imaging the specimen at that location. The volume imaging apparatus of the invention allows for rapid scanning of an entire microscope slide at high image resolution.

Appareil et procédé associé pour l'imagerie en continu du volume d'un échantillon. Ledit procédé consiste à produire des images d'une pluralité de plans d'images à des profondeurs focales prédéterminées. La profondeur focale pour chaque plan d'image est réglable selon toute une gamme de configurations spatiales désirées. Les images produites aux différents plans sont traitées en parallèle et l'image correspondant à une profondeur focale optimale d'une partie de l'échantillon est sélectionnée pour la production de l'image de l'échantillon correspondant à cette partie. Ledit appareil d'imagerie de volume permet un balayage rapide d'une lamelle entière de microscope à une résolution d'image élevée.

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