Automatic process for purifying physiologically active...

Details Automatic process for purifying physiologically active... Automatic process for purifying physiologically active...

C - Chemistry, Metallurgy

12

N

167/139, 23/375,

C12N 9/00 (2006.01) C07K 1/36 (2006.01) C07K 14/525 (2006.01) C07K 14/555 (2006.01)

Patent

CA 1286696

ABSTRACT OF THE DISCLOSURE An automatic process for isolating and purify- ing physiologically active substances contained in large quantities of sample solutions, which comprises the following three steps: (1) filtration (a step for removing microbial cells and other insoluble matters), (2) ultrafiltration (a step for removing impurity having a molecular weight lower than that of the active substance, and concentrating the sample solution), and (3) affinity chromatography using a carrier with a substance having specific affinity to the active substance fixed thereto (a step for isolating pure product by selective adsorption and elution), an apparaturs for use in the same and a carrier employed in the same.

539934

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