Autoradiographic gene-screening method

Details Autoradiographic gene-screening method Autoradiographic gene-screening method

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01)

Patent

CA 1228002

AUTORADIOGRAPHIC GENE-SCREENING METHOD ABSTRACT OF THE DISCLOSURE An autoradiographic gene-screening method employing a hybridization process, which comprises: (1) a step of transferring at least a portion of nucleic acids, fragments thereof or derivatives thereof resolved on a medium onto a transfer support to fix them thereonto; (2) a step of hybridizing the nucleic acids, frag- ments thereof or derivatives thereof fixed onto said transfer support with radioactively labeled probes; and (3) a step of obtaining locational information on the radioactively labeled substances on said transfer support, which comprises placing said transfer support having been subjected to the hybridization and a stimu- lable phosphor sheet in layers for a given period of time to cause said sheet to absorb at least a portion of radi- ation energy emitted by the radioactively labeled sub- stances on said transfer support, exciting said stimula- ble phosphor sheet with an electromagnetic wave to re- lease the radiation energy stored in said sheet as stimu- lated emission, and detecting the stimulated emission.

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