Autosampler for high-performance liquid chromatography

G - Physics – 01 – N

Patent

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Details

G01N 30/22 (2006.01) G01N 30/02 (2006.01)

Patent

CA 2645453

The invention relates to an autosampler for high-performance liquid chromatography (HPLC) with a high-pressure injection valve that has a considerably improved service life in comparison to the prior art, particularly in operation at very high pressures. This objective is achieved according to the invention by adapting the geometry of the valve components in such a manner and establishing the connections of the high-pressure injection valve in such a manner that the switching processes do not take place in a direction that reduces the service life. The grooves in rotor and/or the port opening cross sections in stator and the rotational direction are selected such that fluid flows from grooves under high pressure in the direction of narrow, substantially pressure-free ports are avoided. This applies in particular to the switching process from INJECT to LOAD, since the sample loop contains a relatively large dead volume of compressed fluid. In addition, the valve can be controlled according to the invention in such a manner that an appropriate time is available for reducing harmful or undesired pressure differences. The service life of a high-pressure injection valve according to the invention is limited only by the unavoidable abrasion between rotor and stator and possibly by the abrasive effect of particles such as contaminants or sample materials.

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