Avian influenza virus antigen, and booster immunization...

A - Human Necessities – 61 – K

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A61K 39/145 (2006.01) A61K 39/39 (2006.01) A61P 31/16 (2006.01)

Patent

CA 2734643

A recombinant HA antigen and a recombinant NP antigen are produced by gene recombination techniques. The recombinant HA antigen, the recombinant NP antigen and a synthetic oligo-CpG, which is a mucosal adjuvant, are administered orally to a chicken which has received the intramuscular administration of an oil vaccine at that point in time when the ELISA antibody titer in the chicken is decreased. As a result, the antibody titer is induced again by the oral administration of a mixture of the recombinant HA antigen, the recombinant NP antigen and the synthetic oligo-CpG even though the immune response induced by the oil vaccine is attenuated.

L'invention porte sur un antigène HA recombinant et sur un antigène NP recombinant qui sont produits par des techniques de recombinaison géniques. L'antigène HA recombinant, l'antigène NP recombinant et un oligo-CpG synthétique, qui est un adjuvant muqueux, sont administrés par voie orale à un poulet qui a reçu l'administration intramusculaire d'un vaccin à base d'huile à l'instant dans le temps où le titre d'anticorps ELISA dans le poulet a diminué. Il en résulte que le titre d'anticorps est induit à nouveau par l'administration orale d'un mélange de l'antigène HA recombinant, de l'antigène NP recombinant et de l'oligo-CpG synthétique, même si la réponse immunitaire induite par le vaccin à base d'huile est atténuée.

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