Avian pluripotent embryonic germ cell line

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/00 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2359775

Disclosed in this invention is a process for preparing an established avian embryonic germ cell line comprising the steps of: (a) culturing primordial germ cells (PGCs) isolated from an avian embryonic gonad in a medium supplemented with a cell growth factor and a differentiation inhibitory factor to obtain EG cell colonies; (b) culturing the EG cells in the same medium as in step (a) by employing a feeder layer until the EG cells are colonized; and (c) recovering and subculturing the EG cells in the same medium as in step (a) to establish the EG cell line.

L'invention concerne un procédé d'élaboration d'une lignée de cellules germinales embryonnaires aviaires établie, qui comporte les étapes consistant à: a) mettre en culture des cellules germinales embryonnaires isolées à partir d'une gonade embryonnaire aviaire dans un milieu auquel a été ajouté un facteur de croissance cellulaire et un facteur inhibant la différenciation en vue d'obtenir des colonies de cellules germinales embryonnaires; b) mettre en culture les cellules germinales embryonnaires dans le même milieu que celui mentionné à l'étape a) en utilisant une couche de nutriment jusqu'à ce que les cellules germinales embryonnaires constituent des colonies; et c) récupérer et mettre en sous-culture les cellules germinales embryonnaires dans le même milieu que celui mentionné à l'étape a) pour établir la lignée de cellules germinales embryonnaires.

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