C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) A01K 67/027 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2300380
A culture system for maintaining avian PGCs for long periods in tissue culture is provided. This culture system uses LIF, bFGF, IGF-I and SCF. The resultant PGCs are useful for the production of transgenic and chimeric avians, in particular, chickens or turkeys.
L'invention concerne un système de culture permettant la mise en culture tissulaire prolongée de cellules sexuelles primordiales d'oiseau. Le système de culture considéré fait appel aux facteurs suivants : facteur inhibiteur des leucocytes (LIF), facteur basique de croissance des fibroblastes (bFGF), facteur I de croissance insuline-like (IGF-I) et facteur des cellules souches (SCF). Les cellules sexuelles primordiales résultantes sont utiles pour la production d'oiseaux transgéniques et chimères (en particulier, poulets et dindes).
Blackwell Catherine
Gao Xiu Ying
Jerry D. Joseph
Ponce de Leon F. Abel
Robl James M.
Gowling Lafleur Henderson Llp
University Of Massachusetts A. Public Institution Of Higher Educ As Represented By Its Amherst Campus
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