G - Physics – 01 – N
Patent
G - Physics
01
N
150/3, 167/44
G01N 33/566 (2006.01) G01N 33/541 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01) G01N 33/76 (2006.01)
Patent
CA 1312277
-0- AVIDIN- AND BIOTIN-IMMOBILIZED REAGENTS, ANALYTICAL ELEMENTS AND METHODS OF USE Abstract of the Disclosure Reagents have been prepared from water- insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin deriv- ative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analy- tical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a com- pound of biological interest.
567821
Burdick Brent A.
Danielson Susan J.
Mcclune Gregory J.
Snyder Sean
Sutton Richard C.
Burdick Brent A.
Danielson Susan J.
Eastman Kodak Company
Gowling Lafleur Henderson Llp
Mcclune Gregory J.
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