Axmi-027, axmi-036 and axmi-038, a family of delta-endotoxin...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/32 (2006.01) A01H 5/10 (2006.01) A01N 63/02 (2006.01) C07K 14/195 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/31 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2601857

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NOS: 2, 11 and 13, or the nucleotide sequences set forth in SEQ ID NOS: 1, 10 and 12, as well as variants and fragments thereof.

L'invention concerne des compositions et des procédés destinés à conférer une activité pesticide à des bactéries, des plantes, des cellules végétales, des tissus et des graines. L'invention concerne également des compositions comportant une séquence de codage d'un polypeptide de delta-endotoxine. Les séquences de codage peuvent être employées dans des structures ou des cassettes d'expression d'ADN pour la transformation et l'expression dans des plantes et des bactéries. Lesdites compositions contiennent également des bactéries, des plantes, des cellules végétales, des tissus et des graines transformés. L'invention concerne notamment des molécules d'acides nucléiques de delta-endotoxine isolés. L'invention concerne également des séquences d'acides aminés correspondant aux polynucléotides. L'invention concerne également des molécules d'acides nucléiques isolés contenant des séquences de nucléotides codant la séquence d'acides aminés indiquées dans SEQ ID NOS: 2, 11 et 13, ou les séquences de nucléotides indiquées dans SEQ ID NOS: 1, 10 et 12, ainsi que des fragments et variants de celles-ci.

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