Axmi-115, axmi-113, axmi-005, axmi-163 and axmi-184:...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/325 (2006.01) A01H 5/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2728622

Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or ex-pression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particu-lar, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present in-vention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO: 1, 2, 3, 11, or 12, as well as variants and frag-ments thereof.

La présente invention concerne des compositions et des procédés destinés à conférer une activité insecticide à des cellules hôtes. L'invention concerne également des compositions comprenant une séquence codante pour un polypeptide delta-endotoxine. Les séquences codantes peuvent être utilisées dans des constructions d'ADN ou des cassettes d'expression pour être transformées et exprimées dans des cellules hôtes. Les compositions comprennent également des cellules hôtes transformées. En particulier, l'invention concerne des molécules d'acides nucléiques delta-endotoxine isolées. De plus, l'invention concerne les séquences d'acides aminés correspondant aux polynucléotides, et les anticorps se liant spécifiquement à ces séquences d'acides aminés. En particulier, la présente invention concerne des molécules d'acides nucléiques isolées comprenant des séquences de nucléotides codant pour la séquence d'acides nucléiques montrée dans la SEQ ID No. : 4, 5, 6, 13, ou 14, ou la séquence de nucléotides montrée dans la SEQ ID No. : 1, 2, 3, 11, ou 12, ainsi que des variants et des fragments de celles-ci.

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