Axmi-150 delta-endotoxin gene and methods for its use

Details Axmi-150 delta-endotoxin gene and methods for its use Axmi-150 delta-endotoxin gene and methods for its use

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C07K 14/325 (2006.01)

Patent

CA 2747826

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules having nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 4, 5, 6, 7, 8, 9, 10, 11, or 12, as well as variants and fragments there-of.

Cette invention concerne des compositions et des procédés permettant de conférer une activité pesticide à des bactéries, des plantes, des cellules végétales, des tissus et des semences. L'invention décrit des compositions comprenant une séquence codant des polypeptides pesticides. Lesdites séquences codantes peuvent être utilisées dans des constructions d'ADN ou des cassettes d'expression pour permettre la transformation et leur expression chez les plantes et les bactéries. Les compositions selon l'invention comprennent également les bactéries, les plantes, les cellules végétales, les tissus et les semences transformés. L'invention décrit en particulier des molécules isolées d'acides nucléiques de pesticides, en plus des séquences d'acides aminés correspondant aux polynucléotides. La présente invention concerne, en particulier, des molécules isolées d'acides nucléiques ayant des séquences nucléotidiques codant la séquence d'acides aminés exposée dans SEQ ID No. :2, la séquence nucléotidique exposée dans SEQ ID No. : 1, 3, 4, 5, 6, 7, 8, 9, 10, 11 ou 12, ainsi que des variants et fragments de celles-ci.

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