Bacillus thuringiensis cryet29 compositions toxic to...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) C07K 14/325 (2006.01) C07K 16/12 (2006.01) C12N 1/21 (2006.01) C12N 15/82 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2267667

Disclosed is a novel .delta.-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonopteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

L'invention concerne une nouvelle endotoxine-.delta., dénommée CryET29, qui présente une activité insecticide contre les siphonoptères, notamment la larve de la puce du chat (Ctenocephalides felis), et contre les coléoptères, notamment la larve nuisible aux racines du maïs dans le sud des Etats-Unis (Diabrotica undecimpunctata), la chrysomèle occidentale des racines du maïs (D. virgifera), le doryphore du Colorado (Leptinotarsa decemlineata), le scarabée japonais (Popillia japonica), et le tribolium rouge de la farine (Tribolium castaneum). L'invention concerne également des segments d'acide nucléique codant CryET29, des vecteurs recombinés, des cellules hôtes, et des plantes transgéniques comprenant un segment d'ADN cryET29. L'invention concerne également des procédés de production et d'utilisation des protéines et des segments d'acide nucléique, ainsi que des matériels de diagnostic et de dosage pour détecter les séquences de cryET29 et de cryET29 in vivo et in vitro.

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