Bacillus thuringiensis cryet4 and cryet5 toxin genes and...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/32 (2006.01) A01N 63/00 (2006.01) C07K 14/325 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01)

Patent

CA 2145859

A Bacillus thuringiensis strain isolate, designated B.t. strain EG5847, exhibits insecticidal activity against lepidoptetan insects. Two novel toxin genes from B.t. strain EG5847 designated cryET4 and cryET5 produce insecticidal proteins with activity against a broad spectrum of insects of the older Lepidoptera. The cryET4 gene has a nucleotide base sequence shown in Figure 1 and listed in SEQ ID NO: 1 and produces a cryET4 gene product having the deduced amino acid sequence shown in Figure 1 and listed in SEQ ID NO: 2. The cryET5 gene has a nucleotide base sequence shown in Figure 2 and listed in SEQ ID NO: 3 and produces a cryET5 gene product having the deduced amino acid sequence shown in Figure 2 and listed in SEQ ID NO: 4.

L'invention se rapporte à un isolat d'une souche de Bacillus thuringiensis, appelée souche EG5847 de B.t, qui possède un pouvoir insecticide contre les lépidoptères. Deux nouveaux gènes de toxine provenant de la souche EG5847 de B.t., appelés cryET4 et cryET5, produisent des protéines insecticides agissant contre une large gamme d'insectes de l'ordre des lépidoptères. Le gène cryET4 possède une séquence de bases de nucléotides représentée à la figure 1 et dont la liste se trouve à SEQ ID NO: 1 et il donne un produit génique cryET4 possédant la séquences d'acides aminés déduite représentée à la figure 1 et dont la liste se trouve à SEQ ID NO: 2. Le gène cryET5 possède une séquence de bases de nucléotides représentée à la figure 2 et dont la liste se trouve à SEQ ID NO: 3 et donne un produit génique cryET5 possédant la séquence d'acides aminés déduite représentée à la figure 2 et dont la liste se trouve à SEQ ID NO: 4.

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