Bacillus thuringiensis cryiiic(b) toxin gene and protein...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/32 (2006.01) A01N 37/18 (2006.01) A01N 63/00 (2006.01) C07H 21/04 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2101338

2101338 9213954 PCTABS00014 A Bacillus thuringiensis strain isolate, designated EG5144, exhibits insecticidal activity against coleopteran insects, including Colorado potato beetle and insects of the genus Diabrotica. A novel toxin gene in B.t. strain EG5144 produces an irregularly shaped insecticidal crystal protein of approximately 70 kDa that is toxic to coleopteran insects. The cryIII-type gene (SEQ ID NO:1), designated as the cryIIIC(b) gene, has a nucleotide base sequence illustrated in Figure 1.

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