Bacillus thuringiensis crystal protein hybrids

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01N 63/02 (2006.01) A01P 7/04 (2006.01) C07K 14/325 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2419029

Synthetic nucleotide sequences optimized for expression in plants encode varying forms of the hybrid Bacillus thuringiensis delta-endotoxin H04, the toxin portion of which contains domains I and II of Cry1Ab and domain III of Cry1C. Compositions and formulations containing the insecticidal toxins are capable of controlling insect pests. The invention is further drawn to methods of making the hybrid toxins and to methods of using the nucleotide sequences, for example in microorganisms to control insect pests and in trenasgenic plants to confer insect resistance.

L'invention concerne des séquences nucléotidiques synthétiques, optimisées pour l'expression chez les végétaux, codant plusieurs formes de la delta-endotoxine H04 hybride de Bacillus thuringiensis , la partie de toxine de celle-ci renfermant les domaines I et II de Cry1Ab et le domaine III de Cry1C. L'invention concerne également des compositions et des préparations renfermant les toxines insecticides capables de lutter contre les insectes. L'invention concerne en outre des procédés de préparation des toxines hybrides, ainsi que des procédés d'utilisation des séquences nucléotidiques, par exemple, dans des micro-organismes, en vue de lutter contre les insectes et dans des plantes transgéniques, en vue de conférer à celles-ci une résistance aux insectes.

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