C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/325 (2006.01) C12N 1/00 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2426793
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding .delta.-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
L'invention concerne des acides nucléiques ainsi que des variants et fragments de ceux-ci, obtenus à partir de souches de Bacillus thuringiensis codant des .delta.-endotoxines à activité pesticide contre des parasites de l'ordre Coleoptera. L'invention concerne également des acides nucléiques mutagénisés qui ont été modifiés pour coder des endotoxines ayant une activité pesticide améliorée et/ou une spécificité parasitaire modifiée. Des modes de réalisation particuliers de l'invention concernent des acides nucléiques isolés codant des protéines pesticides, des compositions pesticides, des cassettes d'expression, ainsi que des micro-organismes et des plantes transformés comportant un acide nucléique selon l'invention. Ces compositions sont utilisées dans des procédés d'élimination des parasites, en particulier des parasites de plantes.
Abad Andre R.
Duck Nicholas B.
Feng Xiang
Flannagan Ronald D.
Kahn Theodore W.
E.i. Du Pont de Nemours And Company
Smart & Biggar
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