Bacillus thuringiensis strain, method for their isolation...

Details Bacillus thuringiensis strain, method for their isolation... Bacillus thuringiensis strain, method for their isolation...

C - Chemistry, Metallurgy

12

N

167/5.1, 195/1.3

C12N 1/20 (2006.01) A01N 63/00 (2006.01) C07K 14/325 (2006.01)

Patent

CA 1328239

ABSTRACT An insecticidal composition comprising at least one of the bacteria selected from Bacillus thuringiensis strains identified as NRRL deposits under accession numbers as: Image The invention also provides a method for producing a Bacillus thuringiensis having selective insecticidal activity comprising: (a) providing a first Bacillus thuringiensis strain, having a specific insecticidal activity conferred by a gene coding for an insecticidal toxin protein, the gene being located on a plasmid, in admixture with an intermediate Bacillus re- cipient strain whereby the intermediate Bacillus recipient strain acquires by conjugation the plasmid conferring insec- ticidal activity; (b) isolating and identifying the intermediate Bacillus recipient strain which has acquired the plasmid conferring insecticidal activity; (c) providing the transconjugant intermediate Bacillus recipient strain isolated in step (b) in admixture with a second Bacillus thuringiensis strain whereby the second Bacillus thuringiensis strain acquires the plasmid conferring insecticidal activity from the transconjugant intermediate Bacillus recipient strain and (d) isolating and identifying a transconjugant from the culture admixture of step (c), having selectively targeted insecticidal activity.

565707

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