Bacillus thuringiensis strains and their genes encoding...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01N 63/00 (2006.01) A01N 63/02 (2006.01) C07K 14/325 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/82 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2079877

Two new Bacillus thuringiensis strains, which are deposited at the DSM under accession nos 5870 and 5871, produce new crystal proteins during sporulation that are toxic to Coleoptera and that are encoded by new genes. The crystal proteins contain protoxins, which can yield toxins as trypsin-digestion products. A plant, the genome of which is transformed with a DNA se- quence that comes from either one of the strains and that encodes an insecticidally effective portion of its respective protoxin or encodes its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin, toxin and/or insecticidally effective protoxin portion can be used as the active ingredient in an insecticidal composition for combatting Cole- optera.

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