Bacillus thuringiensis transposon tn5401 and its use in a...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/75 (2006.01) A01N 63/00 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 9/90 (2006.01) C12N 15/32 (2006.01) C12N 15/54 (2006.01) C12N 15/61 (2006.01)

Patent

CA 2166691

A transposable element, or transposon, isolated from Bacillus thuringiensis (B.t.) and designated as transposon Tn5401. The invention also includes a method of using this transposon in a site-specific recombination system for construction of recombinant B.t. strains that contain insecticidal B.t. toxin protein genes and that are free of DNA not native to B.t..

L'invention concerne un élément transposable, ou transposon, isolé à partir du Bacillus thuringiensis (B.t.) et appelé transposon Tn5401. Cette invention concerne également une méthode permettant d'utiliser ce transposon dans un système de recombinaison sitospécifique afin d'élaborer des souches de B.t. recombinées qui contiennent les gènes protéiques d'une toxine de B.t. insecticide, et qui soient dépourvues d'ADN non natif du B.t..

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