C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/90 (2006.01)
Patent
CA 2418317
The present invention provides methods for building DNA constructs in vitro, transforming such constructs into competent Bacillus strains with good efficiency, and generating populations of mutants. Also provided is a method to assemble DNA constructs in situ.
La présente invention concerne des méthodes d'élaboration de gènes hybrides d'ADN <i>in vitro</i>, de transformation de telles constructions en souches de bacille compétentes de manière efficace, et de génération de populations de mutants. Cette invention a également trait à une méthode d'assemblage de gènes hybrides d'ADN <i>in situ</i>.
Diaz-Torres Maria R.
Lee Edwin W.
Morrison Thomas B.
Schellenberger Volker
Selifonova Olga V.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
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