C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/20 (2006.01) A01N 63/02 (2006.01) C05F 11/08 (2006.01)
Patent
CA 2467441
The invention concerns a method for obtaining in vitro bacterial biomasses of edaphic origin comprising steps which consist in: contacting an aqueous soil suspension with a matrix substrate, so as to form a hydrated zone, or biofilm, at the surface of the substrate, similar to the one adjacent to the clay-humus complex of the soil, essentially containing the internal bacterial constituent of the soil, so as to retain at the surface of the matrix substrate, the essential part of the bacterial flora endogenous to the original edaphic media; maturing the biofilm providing oligotrophic conditions enabling the cells which constitute most of the edaphic biofilm which have colonized the matrix substrate to migrate to the supernatant liquid phase of the matrix substrate, and gradually to prevail over it; recovering and culturing the bacterial strains most prolific in rich liquid culture media, with internal character, rustic and persistent in situ, capable of being cultured in liquid environment, hence capable of industrial production. The resulting bacterial biomasses and strains are useful for microbiological bacterization of soils and crop residues, in particular cereal crop residues, including those of corn.
L'invention vise un procédé d'obtention in vitro de biomasses bactériennes d'origine édaphique comprenant les étapes de : - mise en contact d'une suspension aqueuse de sol avec un substrat matriciel, de manière à former une zone hydratée, ou biofilm, à la surface du substrat, analogue à celle adjointe au CAH du sol, abritant pour l'essentiel la composante bactérienne autochtone du sol, afin de retenir à la surface du substrat matriciel, l'essentiel de la microflore bactérienne endogène aux milieux édaphiques d'origine. - maturation du biofilm avec établissement de conditions oligotrophiques permettant aux cellules les plus constitutives du biofilm édaphique qui ont colonisé le substrat matriciel, de migrer vers la phase liquide surnageant le substrat matriciel, et progressivement à la dominer, - récupération et culture des souches bactériennes les plus prolifiques en milieux de cultures liquides riches, à caractère autochtone, rustiques et persistantes in situ, cultivables en milieux liquides, et donc aptes à être produites industriellement. Application des biomasses bactériennes et souches obtenues à la bactérisation microbiologique de sols et de résidus de cultures, en particulier de résidus de cultures céréalières, y compris ceux du maïs-grain.
Claude Pierre-Philippe
Na
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