Bacterial cell component-unresponsive model mouse

Details Bacterial cell component-unresponsive model mouse Bacterial cell component-unresponsive model mouse

A - Human Necessities

01

K

A01K 67/027 (2006.01) C12N 15/09 (2006.01) C12N 15/85 (2006.01) C12Q 1/00 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) G01N 33/68 (2006.01) C07K 14/705 (2006.01)

Patent

CA 2368217

A knockout mouse which is unresponsive to peptidoglycan, a lipoprotein/lipopeptide and the like, and is useful for elucidating the contribution of individual members of the TLR family to a signaling stimulated with bacterial cell components in vivo, in particular, the role of TLR2 and MyD88 in vivo. A bacterial cell component-unresponsive knockout mouse is generated by a process comprising the steps of: a targeting vector is constructed by replacing a whole or a part of a gene fragment of an exon region containing a cytoplasmic region of TLR2 or MyD88 gene and the like with a plasmid having a poly A signal and a marker gene; the targeting vector is introduced into an embryonic stem cell; the targeting embryonic stem cell having a homologously recombined TLR2 or MyD88 gene is microinjected into the blastocyst of a mouse and the blastocyst is put back into the uterus of a recipient mouse.

Souris knockout ne répondant pas aux peptidoglycanes, lipoprotéines, lipopeptides, etc., qui est utile pour clarifier le rôle de chaque membre de la famille TLR (notamment TLR2 et MyD88) dans la traduction des signaux due à la stimulation par des composants cellulaires bactériens in vivo. Cette souris knockout est préparée par une technique de recombinaison homologue au moyen d'un vecteur de ciblage. Pour construire ledit vecteur, on remplace le gène entier ou une partie dudit gène, au niveau du site d'exon contenant la région intracellulaire de TLR2 ou MyD88, par un plasmide comportant un signal poly(A) et un gène marqueur.

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