Bacterial detection by phage transduction of detectable...

C - Chemistry – Metallurgy – 12 – N

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C12N 7/01 (2006.01) C07K 14/21 (2006.01) C12N 9/24 (2006.01) C12N 15/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/04 (2006.01) C12Q 1/10 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2000159

12176-6-1 BACTERIAL DETECTION BY PHAGE TRANSDUCTION OF DETECTABLE PHENOTYPE Abstract of the Disclosure Viable bacteria may be detected in biological samples by exposing bacterial cultures obtained from the samples to transducing particles having a known host range. Such transducing particles carry a heterologous gene capable of altering the phenotype of the bacteria in a readily detectable manner. For example, the transducing particles may carry an ice nucleation gene and the alteration of phenotype may be detected using an ice nucleation assay. By employing a panel of phage, unknown bacteria may be typed based on the pattern of reactivity observed. C:\WORK\PAT\121766-1.JMH

On peut déceler des bactéries revivifiables dans des prélèvements biologiques en exposant les cultures bactériennes issues de ces prélèvements à des particules transductrices ayant une gamme d'hôtes connue. Ces particules transductrices portent un gène hétérologue capable de modifier le phénotype des bactéries d'une façon nettement décelable. Par exemple, les particules transductrices peuvent porter un gène glaçogène; on peut alors déceler la modification du phénotype à l'aide d'une épreuve permettant de mettre en évidence l'activité glaçogène. En employant un panel de phages, il est possible de typer des bactéries inconnues en fonction du type de réaction observé.

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