Bacterial effector proteins which inhibit programmed cell death

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A61K 35/74 (2006.01) A61K 38/16 (2006.01) C07K 14/195 (2006.01) C07K 14/21 (2006.01) C12N 15/81 (2006.01) C12N 15/82 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2494841

~ The present invention relates to a bacterial effector protein which inhibits programmed cell death in eukaryotes and a nucleic acid molecule encoding such a protein. The present invention also relates to methods of suppressing programmed cell death in eukaryotes, delaying senescence in plants, and increasing protein expression in plants. The present invention further relates to a nucleic acid construct having a nucleic acid molecule encoding a first protein, which suppresses immunity by inhibition of programmed cell death in eukaryotes, coupled to a nucleic acid molecule encoding a second protein which is toxic when expressed in eukaryotes. Additionally, the present invention provides a method of stabilizing a transgenic plant transformed with such a nucleic acid construct. Finally, the present invention provides a method of treating subjects for a condition mediated by programmed cell death involving administering to the subject a bacterial effector protein which inhibits programmed cell death.

La présente invention concerne une protéine effectrice bactérienne qui inhibe l'apoptose chez les eucaryotes et une molécule d'acide nucléique codant pour cette protéine. La présente invention concerne également des méthodes permettant de bloquer l'apoptose chez les eucaryotes, de retarder la sénescence chez les plantes et d'augmenter l'expression des protéines chez les plantes. La présente invention concerne également une séquence d'acide nucléique hybride comprenant une molécule d'acide nucléique codant pour une première protéine, qui bloque l'immunité par inhibition de l'apoptose chez les eucaryotes, couplée à une molécule d'acide nucléique codant pour une seconde protéine qui est toxique lorsqu'elle est exprimée chez les eucaryotes. De plus, la présente invention concerne une méthode permettant de stabiliser une plante transgénique transformée à l'aide de ladite séquence d'acide nucléique hybride. Finalement, la présente invention concerne une méthode destinée au traitement de patients atteints d'un trouble induit par l'apoptose, consistant à administrer au patient une protéine effectrice bactérienne qui inhibe l'apoptose.

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