Bacterial packaging strains usefuel for generation and...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/79 (2006.01) A61K 39/00 (2006.01) C07H 21/02 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 7/01 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/74 (2006.01) C12N 15/87 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2587493

Bacterial packaging strains useful for generating recombinant double-stranded RNA nucleocapsids (rdsRNs) are provided. The packaging strains are useful for the production of RNA encoding vaccine antigens, bioactive proteins, immunoregulatory proteins, antisense RNAs, and catalytic RNAs in eukaryotic cells or tissues. Recombinant ssRNA is introduced into the strains and packaged to form rdsRNs de novo.

L'invention concerne des souches d'encapsidation bactériennes utiles pour générer des nucléocapsides d'ARN bicaténaire recombiné (rdsRN). Les souches d'encapsidation sont utiles pour la production d'un ARN codant pour des antigènes vaccinaux, des protéines bioactives, des protéines immunorégulatrices, des ARN antisens et des ARN catalytiques dans des tissus ou des cellules eucaryotes. Un ssARN recombiné est introduit dans les souches et encapsidé en vue de la formation de rdsRN de novo.

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