C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.29
C12N 15/00 (2006.01) C12P 21/02 (2006.01)
Patent
CA 1213540
BACTERIAL PRODUCTION OF HETEROLOGOUS POLYPEPTIDES UNDER THE CONTROL OF A REPRESSIBLE PROMOTER-OPERATOR Abstract A process for bacterially producing heterologous poly- peptides, particularly those such as human IFN-.beta. that inhibit bacterial growth, in which bacteria that have been transformed to express the heterologous polypeptide under the control of a trp pro- moter-operator are cultivated in a known volume of medium containing an excess of a preferred carbon source such as glucose and a prede- termined amount of tryptophan that corresponds approximately to the amount of tryptophan contained in the bacteria in the volume of medium at a predetermined elevated cellular density, whereby expression of the heterologous polypeptide is substantially repressed until the bacteria grow to approximately the predetermined elevated cellular density and is thereafter automatically derepressed to permit expression of the heterologous polypeptide.
445883
Konrad Michael W.
Mark David F.
Borden Ladner Gervais Llp
Chiron Corporation
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