Bacterial production of hydrophobic polypeptides

Details Bacterial production of hydrophobic polypeptides Bacterial production of hydrophobic polypeptides

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) C07K 14/565 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2195799

Methods for the production of interferon-.beta. polypeptides by bacterial cells, by culturing cells capable of producing IFN-.beta. polypeptide under conditions that induce increased production of IFN-.beta. polypeptide. Such conditions include, for example, low potassium cation concentration and/or very low sodium cation concentration and/or specific pH ranges.

L'invention concerne des procédés de production de polypeptides d'interféron- beta à l'aide de cellules bactériennes, en cultivant des cellules capables de produire un polypeptide IFN- beta dans des conditions qui induisent une production accrue du polypeptide IFN- beta . De telles conditions comprennent, par exemple, une faible concentration de cations de potassium et/ou une très faible concentration de cations de sodium et/ou des plages spécifiques de pH.

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