Bacterial protein with xylanase activity

Details Bacterial protein with xylanase activity Bacterial protein with xylanase activity

C - Chemistry, Metallurgy

12

N

C12N 9/42 (2006.01) A21D 2/26 (2006.01) A23K 1/165 (2006.01) C12N 1/20 (2006.01) C12N 9/24 (2006.01) C12S 3/08 (2006.01) D21C 5/00 (2006.01) D21C 5/02 (2006.01) D21C 9/10 (2006.01)

Patent

CA 2203905

The invention relates to specific bacterium and proteins with xylanase activity derived from the bacteria, in particular to xylanases which are free of any significant cellulase activity and which are active at high temperature and at neutral to alkaline pH. Xylanases having these characteristics are particularly useful in the bleaching of wood pulps, such as waft pulps. The preferred bacterium designated B230 was isolated from whire-rotted kerri wood in Western Australia; a sample of which has been deposited under the provision of the Budapest Treaty in the Australian Government Analytical Laboratories under the accession number N94/41262. This preferred bacterium is a gram positive, obligatively aerobic, rod-shaped with a centrally-located spore and has the taxomonic characteristics of Bacillus subtilis (by VITEK method).

La présente invention concerne une bactérie spécifique et des protéines à activité xylanase dérivée de cette bactérie, et en l'occurrence des xylanases qui sont exemptes de toute activité cellulase notoire et sont actives à haute température pour un pH neutre à alcalin. Les xylanases dotées de ces caractéristiques conviennent avantageusement au blanchiment des pâtes à papier telles que les pâtes au sulfate (ou pâtes kraft). La bactérie préférée référencée B230 a été isolée dans la kerrie atteinte de pourriture blanche dans l'état d'Australie Occidentale (Australie). Un exemplaire de cette bactérie a été déposé sous la référence N94/41262 au laboratoire analytique du gouvernement australien (Australian Government Analytical Laboratories) en application des dispositions du Traité de Budapest. Cette bactérie préférée est Gram positive, essentiellement aérobie, bacilliforme à spore centrale et présente les caractéristiques de Bacillus subtilis (selon le procédé VITEK).

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