Bacterial strains for mercury reduction and process for...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/21 (2006.01) B09C 1/10 (2006.01) C02F 3/12 (2006.01) C02F 3/34 (2006.01) C12N 15/52 (2006.01) C12N 15/78 (2006.01) C12N 15/90 (2006.01) A62D 3/00 (2006.01)

Patent

CA 2104502

ABSTRACT Mercury, in its divalent cationic form [Hg (II)], is extremely toxic due to its ability to bind sulfhydryl, thioether and imidazole groups and thereby inactivate enzymes. Organic species of mercury are capable of accumulating in the tissues of higher organisms causing systemic diseases. Despite the toxicity of mercury, mercury containing compounds continue to be discharged into the biosphere. The present invention offers a solution to this problem by providing Pseudomonas Putida strains with heightened mercurial detoxification properties by constitu- tive hyperexpression of the merTPAB genes. The invention also genetically combines the ability to reduce Hg at increased rates under conditions of high concentration, with a benzene degrada- tive pathway. Derivative Pseudomonas Putida strains are thus able to dissociate the chemically dissimilar components of an organomercurial compound, phenylmercuric acetate (PMA) into its metal and aromatic elements, and separately detoxify each. Characterization of these isolates shows that they have potential for treatment of Hg-containing pollutants.

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