Bacterial test method by glycated label binding

Details Bacterial test method by glycated label binding Bacterial test method by glycated label binding

C - Chemistry, Metallurgy

12

Q

C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/28 (2006.01) C12Q 1/70 (2006.01) G01N 33/53 (2006.01) G01N 33/537 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/554 (2006.01) G01N 33/567 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2489230

A method for measuring the bacteria content of fluids such as urine and blood, in which a glycoprotein or glycopeptide is attached to the bacteria and a label attached to or inherent to the glycoprotein or glycopeptide provides a means for determining the amount of bacteria present. A preferred glycoprotein is alkaline phosphatase, which is an enzyme capable of attaching to all bacteria present in the fluid sample and inherently includes a label moiety in that color can be developed by addition of known reagents.

L'invention concerne une méthode de mesure du contenu bactérien de fluides, tels que l'urine et le sang, dans lequel une glycoprotéine ou un glycopeptide est attaché aux bactéries et un traceur attaché ou inhérent à la glycoprotéine ou au glycopeptide fournit un moyen de déterminer la quantité de bactéries présentes. Une glycoprotéine préférée est une phosphatase alcaline qui est une enzyme capable de s'attacher à toutes les bactéries présentes dans l'échantillon de fluide et qui contient de manière inhérente un groupe caractéristique de traceur, dans lequel la couleur peut être développée par addition de réactifs connus.

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