Bacterial vectors

Details Bacterial vectors Bacterial vectors

C - Chemistry, Metallurgy

12

N

530/13, 195/1.2,

C12N 15/63 (2006.01) C07K 14/195 (2006.01) C07K 14/315 (2006.01) C07K 14/815 (2006.01) C12N 15/12 (2006.01) C12N 15/74 (2006.01) C12N 15/75 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2038706

4-17994/A Bacterial Vectors Abstract of the Disclosure The invention relates to the field of genetic engineering and provides novel DNAmolecules which comprise multifunctional origins of replication and/or a DNA sequence coding for the most abundant protein in the supernatant of cultures of Lactococcus spec., named Major Secretion Product (MSP), for its signal peptide and/or the promoter of the MSP gene. The novel DNA molecules are used for the production of novel shuttle vectors for cloning of DNA in at least E. coli and Lactococcus spec. or novel vectors for the expression of homologous or heterologous genes and the production of secreted gene products in gram positive bacteria such as Lactococcus spec. and Bacillus spec.

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