Bacterially-derived, intact minicells that encompass...

Details Bacterially-derived, intact minicells that encompass... Bacterially-derived, intact minicells that encompass...

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A61K 48/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2682704

Intact, bacterially-derived minicells can safely introduce therapeutically effective amounts of plasmid-free functional nucleic acid to target mammalian cells. To this end, functional nucleic acid can be packaged into intact minicells directly, without resort to expression constructs, the expression machinery of the host cell, harsh chemicals or electroporation.

L'invention porte sur des minicellules intactes d'origine bactérienne qui peuvent introduire de façon sûre des quantités thérapeutiquement efficaces d'acide nucléique fonctionnel exempt de plasmide dans des cellules de mammifère cibles. A cette fin, l'acide nucléique fonctionnel peut être directement conditionné dans des minicellules intactes, sans requérir de produits de construction d'expression, de machinerie d'expression de la cellule hôte, de produits chimiques durs ni d'électroporation.

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