Bacteriocins produced by ruminal bacteria

Details Bacteriocins produced by ruminal bacteria Bacteriocins produced by ruminal bacteria

C - Chemistry, Metallurgy

12

N

C12N 1/20 (2006.01) A61K 38/16 (2006.01) C07K 14/195 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2215345

The invention relates to proteinaceous antibiotics, known as bacteriocins, that are produced by ruminal bacteria selected from Butyrivibrio spp. These bacteriocins are generally resistant towards digestion by gastric proteases, may exhibit a high degree of specificity or broad spectrum activity with respect to the microorganisms against which they are effective, and are effective in anaerobic conditions and ineffective in atmospheric conditions. Ruminal isolates capable of producing such bacteriocins include Gram Positive microorganisms characterised by anaerobiosis, the production of butyric acid as a major metabolic end product, resistance to Nalidixic acid, and a vibrioid cellular shape, and are related based on analysis of 16s rRNA gene sequences.

L'invention concerne des antibiotiques protéiniques, appelés bactériocines, que produisent des bactéries ruminales de Butyrivibrio spp. Ces bactériocines résistent généralement à la digestion par les protéases gastriques, elles peuvent avoir un degré élevé de spécificité ou d'activité large spectre par rapport aux micro-organismes contre lesquels elles sont efficaces et, enfin, elles sont efficaces dans un contexte anaérobie et inefficaces dans les conditions atmosphériques. Parmi les isolats ruminaux capables de produire les bactériocines considérées figurent des micro-organismes Gram positifs caractérisés par l'anaérobiose, la production d'acide butyrique comme déchet majeur du métabolisme, la résistance à l'acide nalidixique et une forme cellulaire vibrionienne, et les relations sont établies d'après l'analyse des séquences de gènes ARNr 16s.

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