Banana proteins, dna, and dna regulatory elements associated...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/29 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C07K 14/42 (2006.01) C07K 14/825 (2006.01) C12N 5/10 (2006.01) C12N 9/08 (2006.01) C12N 9/10 (2006.01) C12N 9/24 (2006.01) C12N 15/53 (2006.01) C12N 15/54 (2006.01) C12N 15/55 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2304109

The present invention provides isolated and purified genes which are differentially expressed during banana fruit development, and the protein products of these genes. The present invention further provides DNA regulatory elements which are differentially expressed during banana fruit development, chimeric genes comprising these DNA regulatory elements operably linked to heterologous DNA molecules, and plants transformed with said chimeric genes, providing for controlled expression of said heterologous DNA molecules during the development and ripening of the fruit of said plants, or in response to exogenous ethylene signals in said plants. The present invention also provides a method for expression of a heterologous protein in fruit comprising transforming fruiting plants with one or more chimeric genes according to the present invention, exposing said fruit to an endogenous or exogenous ethylene signal, and harvesting fruit containing said heterologous protein. The method of the present invention may further comprise isolating the proteins produced by said method from the harvested fruit. In a particularly preferred embodiment, the heterologous proteinis a therapeutic protein, which may be isolated from the harvested fruit, or consumed directly in the transformed fruit by a patient in need of said therapeutic protein.

L'invention porte: sur des gènes isolés et purifiés exprimés différentiellement pendant le développement du fruit de la banane, et sur les produits protéiques de ces gènes; sur des éléments régulateurs d'ADN exprimés différentiellement pendant le développement du fruit de la banane, sur des gènes chimères comportant lesdits éléments régulateurs d'ADN fonctionnellement liés à des molécules hétérologues d'ADN pendant le développement et le mûrissement du fruit de ladite plante, ou en réponse à un signal éthylénique exogène dans ladite plante; sur un procédé d'expression d'une protéine hétérologue dans un fruit consistant à transformer les plantes en cours de fructification par un ou plusieurs gènes chimères de l'invention, à soumettre ledit fruit à un signal éthylénique endogène ou exogène, et à récolter le fruit contenant ladite protéine exogène. Le procédé de l'invention peut de plus consister à isoler les protéines ainsi produites des fruits récoltés. Dans l'une des variantes particulièrement préférée, la protéine hétérologue est une protéine thérapeutique pouvant être isolée du fruit récolté ou consommée directement par un patient dans le fruit transformé requérant ladite protéine thérapeutique.

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