Barbiturate assay, tracers, immunogens, antibodies and kit

Details Barbiturate assay, tracers, immunogens, antibodies and kit Barbiturate assay, tracers, immunogens, antibodies and kit

C - Chemistry, Metallurgy

07

D

C07D 405/12 (2006.01) G01N 21/64 (2006.01) G01N 33/52 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/94 (2006.01)

Patent

CA 2042640

The present invention is directed to a fluorescence polarization immunoassay for barbiturates, to the various components needed for preparing and carrying out such an assay, and to methods of making these components. Specifically, tracers, immunogens and antibodies are disclosed, as well as methods for preparing them and a reagent kit containing them. The tracers and the immunogens are made from substituted barbiturate compounds. A fluorescein moiety is included in the tracer, while a poly(amino acid) forms a part of the immunogen. The assay is conducted by measuring the degree of polarization retention of plane-polarized light that has been passed through a sample containing antiserum and tracer. More specifically, the tracers have the following structure: (see above formulas)

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