C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/01 (2006.01) C12N 15/10 (2006.01) C12Q 1/02 (2006.01) G01N 33/50 (2006.01)
Patent
CA 2398690
The present invention relates to methods of using synthetic lethal screening techniques to identify drug targets. The methods of the present invention use "barcoded" libraries of cells, where the library consists of a collection of different mutant clones, each mutant clone bearing a knock-out mutation of a different gene. Each mutant clone has a unique DNA identifier tag, or "barcode", to allow for quick and convenient identification of the clone and its mutation. The use of such a library allows for rapid, quantitative, sensitive and simple identification of genes which interact with a mutated target gene. So identified genes are promising targets for drug screening.
La présente invention concerne des méthodes qui font intervenir des techniques de criblage létal synthétique pour l'identification de cibles de médicaments. On utilise ici des bibliothèques de cellules <= à code barre >= composée d'une collection de différentsclones mutants qui portent chacun une mutation par inactivation d'un gène différent. Chaque clone mutant comporte un identifiant ADN, ou <= code barre >=, qui permet d'identifier rapidement et sans peine le clone et sa mutation. Grâce à une telle bibliothèque, on dispose d'un moyen rapide, quantitatif, sensible et simple d'identification de gènes qui interagissent avec un gène cible mutant. Les gènes ainsi identifiés constituent des cibles prometteuses pour le criblage de médicaments.
Brenner Charles M.
Shoemaker Daniel D.
Osler Hoskin & Harcourt Llp
Rosetta Inpharmatics Inc.
Thomas Jefferson University
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