Bead array reader based-hemagglutination and...

G - Physics – 01 – N

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G01N 33/50 (2006.01) C12Q 1/02 (2006.01)

Patent

CA 2729123

Hemagglutination assays and hemagglutination inhibition assays were introduced in medical and virology practice more than 60 years ago. Since then, these assays have become important tools for measuring concentrations and strengths of viral cultures, the efficacy of the anti-viral immunization, and for studying the neutralizing capacity of virus-specific antibodies. The present invention comprises an improved hemagglutination inhibition assay (HAI), with at least about a 10-fold increase in sensitivity versus the traditional the HAI, to provide more accurate measurements of components in, for example, fluids from the in vitro MIMIC ® system when assessing the effects of anti-viral vaccines (e.g., for seasonal influenza).

Les tests de réaction d'hémagglutination et d'inhibition d'hémagglutination ont été introduits dans la pratique médicale et virologique il y a plus de 60 ans. Depuis lors, ces tests sont devenus des outils importants pour mesurer la concentration et la résistance de cultures virales, l'efficacité de l'immunisation antivirale et pour étudier la capacité de neutralisation d'anticorps spécifiques de virus. L'invention comprend un test de réaction d'inhibition d'hémagglutination (HAI) amélioré, qui présente une sensibilité au moins environ 10 fois supérieure à celle du test classique HAI et permet d'obtenir des mesures plus précises de composants présents, par exemple, dans des fluides provenant du système MIMIC® in vitro lors de l'évaluation des effets de vaccins antiviraux (p. ex. pour la grippe saisonnière).

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