Bead based receptor biology

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/02 (2006.01) A61K 31/7105 (2006.01) A61K 38/17 (2006.01) C07K 1/22 (2006.01) C07K 14/705 (2006.01) G01N 33/52 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/58 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2671310

A method for capturing activated receptor signaling complexes from live cells, utilizing bead based biology wherein live cells are contacted with ligand coated beads to form bead binding sites and thereby initiating formation of a ligand-receptor complex at said bead binding site; and a process for distinguishing and confirming non-specifically bound proteins from specifically bound receptor complexes by utilization of one or more methods of biochemical or biophysical analysis, thereby providing, in a preferred embodiment, a utilization of confocal microscopy and proteomic mass spectroscopy.

L'invention concerne un procédé pour capturer des complexes de signalisation de récepteurs activés à partir de cellules vivantes, à l'aide de la biologie à base de billes. Les cellules vivantes sont mises en contact avec des billes revêtues de ligand pour former des sites de liaison de bille et par là amorcer la formation d'un complexe ligand-récepteur au niveau dudit site de liaison de bille. L'invention concerne également un procédé pour distinguer et confirmer des protéines liées de façon non spécifique vis-à-vis de complexes de récepteurs liés de façon spécifique à l'aide d'un ou plusieurs procédés d'analyse biochimique ou biophysique, fournissant ainsi, dans un mode de réalisation préféré, une utilisation de la microscopie confocale et de la spectroscopie de masse protéomique.

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