Bead emulsion nucleic acid amplification

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2513535

Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.

Cette invention concerne des méthodes d'amplification d'acides nucléiques, lesquelles méthodes consistent à émulsionner des matrices d'acides nucléiques, des billes et une solution de réaction d'amplification et à amplifier les matrices d'acides nucléiques afin que des copies clonales des matrices d'acides nucléiques fixées aux billes soient produites. Cette invention concerne également des trousses et des appareils servant à mettre en oeuvre les méthodes de cette invention.

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