.beta.-fructofuranosidase, its preparation and uses

C - Chemistry – Metallurgy – 12 – N

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C12N 9/26 (2006.01) C07H 3/00 (2006.01) C12N 1/20 (2006.01) C12N 9/24 (2006.01) C12P 19/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2192150

A .beta.-fructofuranosidase with a molecular weight of 49,000~5,000 daltons on SDS-PAGE, an isoelectric point of 4.6~0.5, an optimum pH of about 5.5-6.0, and an optimum temperature of about 50°C in the presence of calcium ion. The enzyme acts on saccharides with a .beta.-fructofuranosidic linkage and other substances including other saccharides, sugar alcohols, and alcohols to produce fructosyl-transferred saccharides in a relatively high yield.

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