C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/42 (2006.01) C12N 9/24 (2006.01) C12N 15/81 (2006.01)
Patent
CA 2736602
The present invention relates to the expression and optimization of enzymes involved in lignocellulosic biomass decomposition. The present invention relates more specifically to beta-glucosidase variants comprising at least one deterioration among the amino acids located at positions 225, 238, 240, and 241 according to the numbering in SEQ ID NO:2 of beta-glucosidase for Trichoderma reesei, and also relates to the use of said variants, having improved performance, in methods for cellulose decomposition and biofuel production.
La présente invention porte sur l'expression et l'optimisation d'enzymes impliquées dans la dégradation de la biomasse lignocellulosique. La présente invention porte plus particulièrement sur des variants de la beta-glucosidase comportant au moins une altération parmi les acides aminés situés en position 225, 238, 240 et 241 selon la numérotation de la SEQ ID :2 de la beta-glucosidase de Trichoderma reesei, ainsi que sur l'utilisation de ces variants à performance améliorée dans des procédés de dégradation de la cellulose et de production de biocarburant.
Fourage Laurent
Lopes-Ferreira Nicolas
Margeot Antoine
Mathis Hugues
Goudreau Gage Dubuc
Ifp Energies Nouvelles
Proteus
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