.beta.-ketoacyl-acp synthetase ii enzymes and genes coding...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2278923

The object of the present invention is to provide a gene of a protein having an enzyme activity which makes it possible to regulate or control the content of saturated fatty acids and unsaturated fatty acids in plant cells, and an enzyme protein as the expression product. There is disclosed a protein with a KASII enzyme activity which has a specific amino acid sequence, typically the amino acid sequence of a .beta.-ketoacyl-ACP synthetase II (KASII) enzyme protein derived from cyanobacterium (Anacystis nidulans) or substantially the same amino acid sequence as the one described above. There is also disclosed a KASII enzyme protein gene coding for the amino acid sequence of the above described protein. Furthermore, there are disclosed a recombinant vector which comprises said gene, and a cell into which said gene has been introduced.

L'invention concerne un gène d'une protéine présentant une activité enzymatique permettant la gestion ou la régulation du contenu d'acides gras saturés et insaturés dans des cellules végétales ainsi que la protéine enzymatique en tant que produit d'expression de ce gène, plus spécifiquement: des protéines présentant une activité beta -cétoacyl-ACP-synthétase II (KAS II) et une séquence d'acides aminés spécifique typifiée par la séquence d'acides aminés de KAS II ayant pour origine des algues bleu-vert (Anacystis nidulans) ou une séquence d'acides aminés sensiblement équivalente; un gène de protéine KAS II codant la séquence d'acides aminés de la protéine précitée et des vecteurs recombinés contenant ce gène et des cellules dans lesquelles ce gène a été transféré.

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