Bifunctional compounds useful in catalyst reporter deposition

Details Bifunctional compounds useful in catalyst reporter deposition Bifunctional compounds useful in catalyst reporter deposition

C - Chemistry, Metallurgy

12

N

C12N 9/16 (2006.01) C07C 245/08 (2006.01) C07C 323/48 (2006.01) C07D 207/46 (2006.01) C07H 13/08 (2006.01)

Patent

CA 2301818

The present invention concerns a method to catalyze reporter deposition to improve detection or quantitation of an analyte in a sample by amplifying the detector signal which comprises immobilizing an analyte dependent enzyme activation system which catalyzes deposition of reporter by activating a conjugate consisting of a detectably labeled substrate specific for the enzyme system, said conjugate reacts with the analyte dependent enzyme activation system to form an activated conjugate which deposits substantially wherever receptor for the activated conjugate is immobilized, said receptor not being reactive with the analyte dependent enzyme activation system. In another embodiment the invention concerns an assay for detecting or quantitating the presence or absence of an analyte in a sample using catalyzed reporter deposition to amplify the reporter signal. Also described are novel compounds which can be used as reagents to prepare HABA-type conjugates.

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