Binary probe and clamp composition and methods for target...

Details Binary probe and clamp composition and methods for target... Binary probe and clamp composition and methods for target...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2360543

Binary probe and clamp compositions conduct methods for target hybridization detection. Where the probe is a substrate for exonuclease cleavage, the composition provides quantitation and detection of PCR products, by real-time and end--point measurements. Where the probe is an amplification primer, the composition provides an improved method for labelling and detection of PCR products. Probes and clamps may be labelled with fluorescent dyes, quenchers, hybridization-stabilizing moieties, chemiluminescent dyes, and affinity ligands. Clamps may be nucleic acid analogs, such as 2-aminoethylglycine PNA.

L'invention concerne des compositions binaires sondes/pinces permettant de mettre en oeuvre des méthodes de détection d'hybridations cible. Lorsque la sonde est un substrat destiné au clivage des exonucléases, la composition permet une quantification et une détection de produits d'amplification PCR, par des mesures en temps réel et des analyses des points terminaux. Lorsque la sonde est une amorce d'amplification, la composition permet de mettre en oeuvre une méthode améliorée de marquage et de détection des produits d'amplification PCR. Les sondes et les pinces précitées peuvent être marquées à l'aide de colorants fluorescents, d'extincteurs de luminescence, de fractions de stabilisation de l'hybridation, de colorants chimiluminescents, et de ligands d'affinité. Les pinces peuvent être des analogues d'acide nucléique, tels que 2-aminoéthylglycine (PNA).

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